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Seasoned punters will find calculating odds to be second nature but for beginners the prospect of getting to grips with what odds mean and in particular grappling with fractional odds can be a daunting one. Even if you're an experienced punter this Masterclass on understanding odds offers you the chance to refresh your thinking on identifying value based on the implied probability of odds. Traditionally, odds in the UK are expressed as a fraction. These odds can be used to calculate your net profit excluding your stake and although they seem complicated the method of calculation is relatively straightforward. This is best explained using an example. So whatever your stake is you can multiply it by 1.

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Hsabet w 39 abetting

Aert van Buchel Advocaet van Wtrecht. Schrifture te kennen gheeft, daer Salomon in den boeck van Pro- verbia feyd : Rex qui fedit in folio Iudicii, deffipat omnc malum intuitu fuo. Ick vinde wel te voeghen,ende uwe Hoogheyd te dienen met dit kort fommier vanfeeruyt-loopende Hiftorie , diemen foude moghen op den gruwel ende verwoeftinghe, dier ghefchiedt zijn, maken.

Uwe Hoogheydt neme voor goet,te bidden ende te perfuaderen fyne Majefteyt, dat hy weygere den ghe- nen die alfulcke fchadelijcke ende boofe eonqueften Tullen begeeren. Iae ghe- biede, belanghende dithelfche verfoeck,eeneeuwigh gefwijgh op alfulcken pene,dat niemand voortaen hem verftoute,niet alleenlijck die te noemen. Columbus bant Hem foo benam boa? Dominicus tot Sevilien: een VueerbigHe mciuoiie boo? Domingo maren berabberteert boo? Cort verhad vande deftrudHe van Indien. En blaeekcren baerdoodt, op Ianghfaem pynlijck vyer?

Dc Rijcken in 't Eylandt Efpannola. De Spaenfche duy veis fnoot, die locken loos met liften. Drie hondert Heeren flccht, in eenen ftroycn Hut, Dces werden door den brand het leven uyt geput. Ancoan die Princes wiert levendigh ghehanghcn, De vluchtelinghen fy als wilde Dieren pranghen.

Uieri rept bat terPiftl be Poo? Al inden Kercker groot, daer hy ghekluyftert fat. G Hy die u Catholijck of Koninghs doet benoemen, Siet hier den fachten aart van u hoovaardich roemen, Ay tiende blindc 3 fict,vvien dat u fotheyt prijft, K Een hoop, een v vreede hoop, daer yeders hart voor yft? JJ ile! O Ghy Hollanders leert,aen dees verheerdeHeden? Pal'ccpccora occifionis,. Dus vvert den Indiaan vvrcetgulfelijek veiflonden J Een vrouwe,. Doch 'thalf ghcvvurchdc wicht, vvert van een Paap ghedoopt.

Ach wat een hclfche daad! Zijnbe alle befe BelftBe tp? Voor vier,vyfthien Millioen heeft willich hy ghegeven. UM;x iv U euyr. Ingham, of the Ber- muda Islands. He was graduated at Yale in , and died in On his mother's side the relationsliip is carried back to Hendrick Van Dyck, who came to New York at an early period, was attorney-general for a time, and died in ; and to Guleyn Vigne, who came from Joseph Jauficey Oiiterhridge Brown.

Henry De Meyer son , born Agnes De Meyer, or Demire daughter , married, , Ed- 1. Lucas Van Tienhoven son , died 1 Cornelius Van Tienhoven son , married 17 Sarah Van Tienhoven, or Tin- over daughter , born , married, , ohn auncey.

Susanna Nicoll daughter , born ; married Joseph Jauncey son. Susanna Jauncey daughter , married John Jauncey Ketchum. Brown always wished to be recognized as an American, saying that as his grandfather was born in Connecticut, and his mother in New York, the accident of his own birth in Bermuda ought not to stamp him as a foreigner. For the iirst thirteen years of his life Mr. Brown lived in Bermuda. During this time his only instruction and training were received from his mother, a lady of refinement and culture.

She, finally, unwilling that he. At her solicitation, her brother, Mr. Edgar Ketchum, who with Mr. Isaac Adriance formed the law firm of Adriance k Ketchum, arranged to take the boy and bring him up "in the nurture and admonition of the Lord. By these means for he was never able to attend school or college he succeeded in fitting himself for llie occupation of iiis life, and in llie young man was admitted to the bar.

Brown continued with his uncle afier the death of the senior member of the firm, and until S. In that year, wishing to assist his only brother, Erastus Fitch Jirown, in the study and jiractice of the law, he left Mr. Ketchum, and established himself in an office at No.

His duties were arduous and wearisome, but in spite of ill-health were pursued with industry and zeal. He adopted the specialty of real- estate law and conveyancing, and his personal knowledge of all the real estate in an important section of this city, his wonderful recollection of landmarks, farm boundaries, old roads, and the like, and of the Rimilies and genealogies of the various owners, and his familiarity with the subtle i In , having attained a considerable degree of success, he decided to retire from active practice ; but the thought of his brother and the desire to aid him in his profession induced a change of purpose.

The year after Mr. Brown's admission to the bar he married Harriet Williams Parsons, a daughter of Dr. Ezekiel W. Parsons, originally of Amherst, Mass. She survives him, as do their four children, Sara Louise Langdon, wife of Mr. Kingsbury, Esq. In the same year, , Mr.

Afterward, in , when the Harlem Congregational Church was formed to give expression to the Christian sentiments that demanded emancipation for the slaves and loyal support to the government in its struggle against treason, he identi- fied himself with that church. He remained a member of that organiza- tion after its removal, in , to its present edifice on Madison Avenue, and until his death. But although duty seemed to prescribe his active connection with these other Christian denominations for a number of years, his love for the Anglican Church, the church of his bapiism in Ber- muda, had never wavered.

He was an authority on all questions relat- ing to its organization, polity, faith, and ritual, of all of which he had ever been a deep and reverent stutlent. His spirit ever sought the rest, not merely of its outward worship, but of its inward and spiritual meaning to the wandering soul, and his feeling found expression in the language of Dr. And so it was natural that during his later years his feet were gladly turned again to St.

Andrew's Protestant Episcopal Church, the first church at which he ever attended divine service in America, and the last. Brown was a true Christian gentleman, with an infinite dignity anil grace of manner that stamped him of sui erior spirit. Of singular purity of character and lofty principles, his innate modcbty prevented him from taking the position to whicli his intellectual attainments entitled him.

Absolutely truth- ful, and yet tenderly careful of the feelings of others, he combined the courtesy of the true gentleman with the dignity of the Christian. His lile was quiet, pure, and unassuming; a man of remarkable sweetness and sincerity of spirit ; and all who were so fortunate as to know him intimately regarded him with deep afl'ec- tion.

Tliat he was a great and wise man, was evident to aU who knew him. He brought a charm and inspiration into this humdrum life of ours, and his individuality was so strong and so interesting that no one could help but admire him. Whatever sorrows burdened him, he never permitted them to impair his genial, kindly nature, and he leaves the precious legacy of a good name and unspotted character.

In his home and professional life he was a conspicuous type of the courtly and dis- tinguished gentleman so rarely met with in these later days. Pretence and narrowness were afar off his spirit. His tenderness to all God's creatures was earnest and delicate. He was ever ready to defend the absent, charitable in his dealings with all men, and possessed of a heart that could overlook the weakness of his fellow-creatures to an extent rarely met with. Brown excelled as a critic in art. His own talent in this direction was of no mean order, and there was a period in his life when he would gladly have given up plod- ding at his profession and devoted himself to that more fascinating pur- suit, in which he would undoubtedly have attained a llattering eminence.

Brown was a life-long lover of nature. He knew every tree, shrub, and flower. His poetic soul saw at a glance the beauties of land- scape, ocean, cloud, river, mountain. And the birds and their different songs were as well known to him, and almost as dear, as his intimate friends. His ear was quick to catch the first tuneful strains of the earliest winged harbingers of spring.

Hearing from a distance the note of a scar- let tanager which he could not see, his own whole landscape would be lighted up to him by the quick imagination of a remote scene some- where, illuminated by the bird's brilliant plumage. Brown was deeply interested in genealogy, and for many years rarely failed to attend the meetings of this Society.

The last evening of his life was spent at his house, in searching in iiis library for facts which he had been asked to look up for a member of the Society. He wrote upon this until half-past eleven o'clock, and at midnight received the summons to end his earthly labors. He was taken from the activities and enjoyments of life here to the better ami higher life beyond — suddenly transhiteil to the home where no sorrow can come, but immortal life glorifies all who enter.

By Riciiaku H. Continued from Vol. I do not know if he was related to John Doughty class of , or if either was connected with the John who was prominent in Brooklyn, N. Erancis Doughty, from Taunton, Mass. We may at least conclude that the graduate, as a loyalist, in the service of the king, retired with the army at the end of the struggle, and there- after ceased to be identified with this stale and nation.

James Ludlow. He was the tenth child of Wni. He was an early merchant in New York, where he arrived November 24, He married Saiah Ilanmer, April 5, They had twelve children. Gabriel' the emigrant was son of Gabriel,' baptized at Warminster, England, August 27, ; he was son of Thomas,' who was first cousin, once removed, of Edmund 16 the Regicitie.

GabrieP and Sarah had three sons, as follows : i. Henry, removed from this city to Claverack, N. Just before the Revolution, his son, Wm. They lived in this city, and left descendants through each of four children, to wit : Daniel, Robert C, Mrs.

Lewis, and Mrs. She was born February 14, , died Se[ teniber 21, William and Mary Duncan Ludlow had a large Ixmily, of which the tenth child, James,' was the graduate. He married, October 22, NoiE. It should be Cassilis. He succeeded to the Scotch earldom of Cassilis late in life.

I Broadway, built by Sir I'eler Warren. He was here in , and that year ] ctitioned the Council for a grant of 2,oot acres in Ulster County, of land formerly granted to Capt. I refer to this because of the error in the name of Earl Cassilis. They had but two children, both daughters : i. Elizabeth," who died young. Philip and Frances Alary Ludlow Thomas had si. Their descendants, of course, comprise all the descendants of the graduate : i.

Elizabeth Frances Thomas. Sarah Margaret Thomas, ' m. Samuel T. Jones, of Philadelphia, iii. Catharine Ann Thomas,' m. Bend, iv. Philip Wm. Thomas' Columbia, , m. Anna C. Martha Mary Thomas,' m. Samuel Tonken Jones. Second wife. Ludlow Thomas' Columbia, 1S35 , m. Mary S. They had Mary Ludlow Thomas. Jones' had Frances Mary," who married, first, Richard M. Pell, and second, Louis T. Catharine A. Bend' had a large family, as follows ; i.

Bend," m. Isabella Tomes, ii. Elizabeth A. Townsend ; they had Amy and Beatrice, iv. Katharine A. James K. Whitaker ; children, Ogden Hoffman and Marion Ludlow. Elizabeth Pelham Bend," m. Henry Asher Robbins. Mary Ludlow Bend. Fanny Thomas Bend," d. Elizabeth P. Robbins' had two children : i. Maud Robbins," who m.

Harry Whitney McVickar. Harry Pelham Robbins. Jones,' m. Jones, and had i. Sarah Margaret Jones," m. Jones," d. Shipley Jones," grad. Elizabeth Ludlow Jones," m. He married, April 20, , Charily, daughter of Maj. Benjamin, the graduate, was son of Lieut. He was son of Samuel and Mary Reed, and his father, Rev. L, till his death in His widow married Francis Doughty, son of Rev. Francis Doughty of Maspeth, alluded to above. The only son of the graduate.

Clement Clark Moore, grad- uated in the class of , Columbia College. William Moore, a younger brother of the graduate of Ctoiiverticur Morris was born January 30, He was half-brother of Lewis Morris, who signed the Declaration of Independence, and graduated at Yale, , before King's College was founded.

Gouverneur succeeded Lewis in the Continental Congress, to LL's life and services are both familiar. He was named after his maternal grand- father, Nicholas Gouverneur, whose daughter Sarah married Colonel Lewis Morris, November 3, , as his second wife. The earliest ancestor in this country was Richard, the governor's father, who came from Kngland to this colony by way of the West Indies, and purchased the tract which gives the name to Morrisania from the Indians.

The subject of his graduation address was " Wit and Beauty. Many of his later writings have been published. There the graduate died, in the same room where he was born si. John, who was born about in this city, but removed to New Jersey. From to was treasurer of New Jersey. He was devoted to mechanical inventions, and, with his brother-in-law, Chancellor Robert R. Livingston , Nicholas I. Roosevelt, and Robert Fulton, did much to perfect and establish steam navigation.

He applied for and received the first charter for a railroad from New York to Philadelphia. He received a patent for railroad construction in In 17S3 he married Rachel, daughter of John Co. John Co. September 24, ; graduated at Columbia, ; m. Maria, daughter of Robert Livingston. Robert Livingston, b.

October 18, ; an eminent steamboat builder and projector of Stevens battery ; unmarried. James Alexander, b. January 29, Columbia, ; ad- mitted to the bar ; was married, had a family. He died in Richard Columbia, ; unmarried; d. Francis Bowes Columbia, ; d.

Edwin Augustus, b. July 28, ; founder of Stevens Institute. Hoboken; d. August's, There were also several daughters. He was member of Assembly, New Y jrk, and twice chosen speaker. They had : i. Maria Cornelia, b. January 25, ; m. William Allen. Eliza Magdalena, b. February 16, ; never married. Anne Sophia, b. September 24, ; d. September 15, David Johnstone, b.

January 18, ; m. Louisa A. Emily, b. January 11, ; m. Claude S. Charlotte de Lancey, b. September 25, ; never married. Gulian, possibly d. Caleb Cooper was the only graduate, and all the catalogue tells us is he received an A. That was the year Piiilip Freneau and President Madison graduated. He came to Lynn, Mass. L One of the children, born in Englantl, , was John," aged 10, afterwards married Sarah , and died His son, Captain John,' wife Hannah, died December 14, I think Caleb," by wife Abigail, had among others a son, Caleb,' Jr.

South Ward, New York city, , thirty-three years after the graduation and fifty-seven years after the birth of Caleb' Cooper. James Creighton. If this is the man who died there in , then it is not the graduate, though it might be the generation before ; but it is possible there is an error in the age, 81, in the death notice. I am convinced his ilisappcarance is to be accounted for in his adherence to the crown and departure with the loyalists. Continentals, January, ; brevet major, September, ; commander-in-chief U.

Army, June, 17S4, to September, , with rank of major; and resigned, iSoo. If Charles, who graduated , was a relative, the loyalty of one would cause us to expect it in the other. We also look for King's College graduates in the English Church, and I find a John Doughty, oltener Doty, officiating in the capacity of lay reader in the summer of in St. Peter's Church, Cortlandt, N. This man was or- d.

The parish of St. Peter's, Peekskill, was organized August 10, Doty became rector July 16, ; his successor was Rev. Bernard Page. He resigned in , and, as was stated above, went to Schenec- tady, where he remained untU , when he obtained liberty to go to Canada, though he was forced to sell his furniture to procure the means for tlie journey. While here he had been twice arrested, and his services had been intermitted on account of iiis loyalty, and after reaching Canada he was chaplain to his Majesty's Royal Regiment at Montreal.

In , however, we find him again at Sorel, Canada, but he resigned in That John was eldest son of Rev. John, a scion of the fam- ily of the Marquis of Montrose, who emigrated to America S. He was born at Exeter, N. Neither institution records the date of Jonathan's death. Richard Harris.

There were merchants of this name in this city for many years before, but I have not connected this man with any of them, and I tiud no Richard Harris in the army of the colonists, so his death was not from that cause. William Hubbard we know was a loyalist, and went to Nova Scotia, where he remained till his death in He was elected to the Assem- bly of the province, and was appointetl chief justice of the Common Pleas, and judge of the Court of Claims.

New York Volunteers, , majur and. Oliver S[ encer's additional Continentals 5th N. October 6, , he was taken prisoner at Port Montgomery. Senator Irom the Eastern District. K , November 4, , to April 5, He was a native of New York city, but removed to Albany at the close of the war. While in New York, in , at the age of 21, he was admitted to the bar, and practised in Albany until his last sickness.

He died April 19, Stephen Lush married Lydia, daughter of Dr. Samuel Stringer, a physician of high standing, a native of Maryland, whence he came to Albany, N. She died August 10, i, aged They had seven children, to wit : i. Samuel Stringer, d. Samuel Stringer, b.

October 20, 17S3 ; m. Ann de Peyster. William, b. November 6, ; m. Margaret Trotter, and d. July 2, 1S Mary, m. Rachel, m. Henry G. Gertrude, m. Ross, vii. Richard, b. June 20, S; d. October 25, 1S2S. Samuel S. He accumulated a large property here, part of which he invested in ihe Van Schaaick farm, on which the farmhouse was located, at tiie junction of Bowery Road and Oliver Street. The sponsors at this baptism were John Provoost and Eva Rutgers, his wife.

She was sister of Mrs. Another grandson of Harman, above, and only surviving son of Hendrick, who was born February 20, , and Catharine do Peyster. John Marshall, the father, married Elsje Rutgers, January 27, I do not think his ancestry can be traced to the New England family, though Mr. Eernow puts Marshall among the English names of residents here before He may be of the family of Marschal, from Amster- dam to Netherlands, Eebruary, , as spelling by clerks often took the form of the writer's nationality.

In March, , a majority of the clergy met at the house of John R. Marshall, rector at Woodbury, Conn. They named Rev. Jeremiah Learning and Rev. Samuel Seabury for the oflice of Bishop. The former declined, and the latter was chosen Bishop of Connecticut and Rhode Island, and continued in that oflice until his death, Philip Pell was born July 7, He inherited Pelham Manor from John Pell, his father, the emigrant ancestor, to whom it was granted by Governor Dongan in In , Philip Pell was commissioned lieutenant and commissary of prisoners; but his name does not appear in the register of Continental officers.

After her death he married Ann Lewis. They had Ward, Philip, and Mary. The graduate Philip Pell was elected member of New York Legis- lature, to and to He was also one of the fust regents of the Universitv, New York State, , and died J XXV. V, Dirk lirinckcihoir, Rlaria Goelet, j.

Isaak Ijiaisier, Aaltje Braisier, j. V Johannes Van Dal- son, Geertje Kily- per. Van A n d r i e s Abraliamse. Willem Colaid, Jan- Willem. Willem Haveland, Jane netje Jacobs. Co lard, h. Van Henry Boman. John Provoost, Eva John. Willem Alexander, Riitgers. Van Elias Parker. Leendert Lispenaar, Alelta. Robert Benson, Maria Elsje Rutgers. Rutger;-, j. Bartholomeiis Schaats, Antje Schaats. Johanna Varik, j. Johannes de Lanov, Jannetje.

A b r a li a m de Lanoy, IMaria Krankheyt. Hester King, syn h. Liikas Mattheman, Elizabeth. C h r i s t o f f e 1 liancker, Catharina Steer. Elizabeth Hoogland, z. Joseph Simons, An- Joseph. Reyuier Hoppe, Ad- natje Hoppe. Arie Koning, Rachel Jenneke.

Gysbert Koning, jen- Kierstede. Jacob lis Bussing, ]acobus. Harmen Bussing, Sara Anna Bisschop. SI over, z. Andries RI i c h i e Is, Andries. Jakob Di'irrie, Catharina Sara Noordstrand. Polhennis, z. David Provoost, Elizabeth. Johannes Valentyn, Johannes.

Frederik Bekker, Maria Maria Pontenier. Meedannel, Wed. Hiiig Foord. Simon Jahnson, Mar- Susanna. Elizabelh Van Home, z. Johannes I'rapliagen, Johannes. Johannes Qiiakken- Cornelia. Albeitus Ticbout, Cor- bos, Margareta nelia Bogart, z. Isaac Bogert, Lea de Jakobiis. Jakobus lie Moree, Mar- Moree. J Thecdorus Van Abraham. Abra- ham Pels. D" Joannes Ritzema, Joannes.

C h ri s to f f e 1 Bancker, Hiltje Dykstra. Tobias Stoutenbiirg, Jan. Pieter Stoi'itenburg, Hen- Neeltje Lansing. Van Jan Stoutenbiirg. Silvester Maniis, Rachel. Maria Mariniis, z. Plendrik Ryke, Elisa- Dorothea Renisse. John Nichols, Jan- Willem. John de Voe. Johannes de Wit, An- Johannes. Jacobus Home, Annatje natje Hers. Zomerendyk, z. Pieter Klopper, Pieter. Abrah'" de Lanoy, Jr. Hcsther King, z. Abraham Brouwer, Petronella. Dorjet, z. Jurrie Leiiw, Susan- Annatje.

Cornel is Cregier, An- na Cregier. Asa King. Ide Van Yveren, Myndert. Yveren, z. Abraham Ciiyler, Hester. Johannes Beekman, jannetje Beekman. Catharina Ten Broek. Willem Stow, Rachel Aaltje. Tibouwt Ecker, Aaltje de Ecker. Jacob Somerendvk, Amarens Stoiiwi.

Johannes Meyer, Aaltje Rome. Nicolas Bicker, Donia de Wit. Johannes Anderson, Vrouwtje Pieters- zon. John Van Horne, Celia de Hart. Petriis Giraud, An- natje Willemsse. Isaac Koning, Geertje Hartje. John Leshy, Maria Holdes.

Mattheus C e n t li s , Elisabeth IMariin. T h e o p h i 1 li s Els- worth, Margareta Se bring. Daniel Van Deiirsen, Lea Hertje. Robert Benson, C a t h ar i n a Van Bossen. John Grauw, Feytje Raleman. Thomas Warneer, Bregtje Aalstein. John Dally, Maria I'. J o r i s Marsclialk, Hester Fyn. Anthony de Milt, Jannetje Raven. Abraham Braser, Jannetje Siggels. A b r a h a m Bokee, Maria Caar.

Samuel Brouwer, Maria Hartje. Egbert Somerendvk, Cor- nelia Dykman, h. Teilns bomerendyk. Yde Mver, Annatje Gcr- rilze, z. Viktoor Bicker, Annatje Cregier, z. John Allen, Ji? Cornells Bogard, Annatje Pel, h. Willem Bogard. Andries Geraiiard, An- natje Burger, z. Vincent de La Alon- tagne, Tryntje Hartje, z. George It'finss, Elisabeth Fan. Francis Martin, Sara Evits, j. Theophilus Elsworlh, Annatje Harden- broek, z.

David Brouwer, Annetje Hertje. Dirk Lefterts, Maria Rut- gers, j. Mattheus Hilisman, Hes- ter Van Zant, z. Leendert Warneer, Catha- lyntje Rappalje, hiiis v. Johannes Aalstein. Johannes de Milt, Gvllje Van der Voort, huis v. Isaac de Milt. Isaac Patrong, Annetje Ecker, z. Isaac Charduvine, Catha- rina Pheni. Joseph Callens, Al- bertje Jansen. Johannes Huygen, Eh'zabet Van Dal- sen. Willem Van Deursen, Catharina Gilbert.

Johannes Elsword, Hesther Rome. Thomas Verdon, J', Margrita Gilbert. Josiia Sliedell, Elisabeth Johnson. Abraham Akkerman, Hendrikje Hoppe. Joliannes Koning, Anna Rovau. Amos Pain, Catharina Bargne. Joh' Adolphus Mer- berg, Annatje Le- quire. Jan de Boog, Vroiiwtje Heyer. G ysbert Koning, Catharina Ram- bler. J Jan. Pieter Remsen, Jan- netje de Hart. Cornelis Quackken- bosch, Annatje Van Home. Walter Heyer, An- natje Echt, 8. Wiert Banta, Annatje Minlhoriie.

Pieter K e te 1 t a s, Elisabet Van Zandt. Anna Mar- grita. Maria Mag- dalena. Gerrit Jansen, Sara Heyer, z. Jan Van Dalsen, Geertje Kiiyper, z. Willem Gilbert, Catha- rina Van Deursen, h. Isaac Van Vlek. Jesse de La IMontagne. Christoffer Elsword, Jo- hanna Haerdin. Thomas Verdon, Aalije Verdon, h. Willem Gilbert. Hendrik Coumen, Rachel Gerritze, z. Hendrik Kermer, Rachel Gerritze, z. Gysbert Koning, Rachel Koning, li. John Hicker. Pieter Bargne, IMargrita Gordon, j. Philip Grim, Anna Mar- grita Rapparin, z.

David Bruyn, Annatje Heyer, j. Jan Koning, Elisabeth Koning, h. John Addissun. Joris Remsen, Geertje de Hart, syn h. Jan de Boogh, Catharina Heyer, syn h. Philip Minthorne, Tan- neke Harsin, syn h. John Keteltas, Catharina Ten Eyck, h. Van Wynand Van Zandt. Jacob Roome, Susanna Charelje, h.

Van Jo- hannes Roome. James Cammel, Sara Sara. Catliarina Hage- woiU. David Dikson, Hel- Jan Ryn. Nicolaas Stillewil, Eiias. Martha Van der Hoef. Johannes Turk, Jo- Elisabet. James Baly, Catha- Jakobus.

Jakob Cool, Maria Sara. Isaac Bertrand, Anna Sar. Reynier Hoppe, Ad- Maria. Pieter Losie, Jannetje Styntje. Andries Gewera, F'. Femmetje de For- eest. John Man, Annatje Ed worth. Louwrens Myer, An- Aaltje. John B e e k m a n , Tlieophilus. Robert Livingston, Margareta. Maria Tang. John Axeen, Maria Annatje. Jc Phenix. Evert Bancker, Eliza- bet Boelen. Jakobus Kip, Eliza- bet Mellens. Edworth Drinkwater, Helena Bur2;er.

Gerardus Hanienbroek, Heyltje Koely, syn h. Samiiel de Moore, Antje de Moore, j. Van Joris Kosaait. Jan Ryn, Helena Broiiwer, Wed. Van John Tournor. Daniel Stillewil, Catlia- rina Van der Hoef, j. Cornelis ] urk, Neeltje Turk, h, v. Van Lau- rens Roome.

Thovet Baly, Hester Van Zant, z. Wiert Banta, Annatje Minthorne, z. John Ekker, Sara Dyer, z. Abraham Huysman, An- natje Hoppe, z. Isaak Koning, Geertje Harlj;', z. Henricus Van de Water, Sara de Foreest, z. Edwoith Mau, Annatje Van Deurssen, j. John Myer, Aaltje Roome, z. Jakob Biuwenton, Adri- aanije Klsworlh, hiiis v. Van Thomas Simour. Philip Livingston, Chris- tina ten Broek, z.

Abraham Bokee, Aalje Teljou, h. Van Tiele- mau Phenix. Christoffel Bancker, Elizabet Hoogland, z. Jacob Ste Vrelanci. Lodewyk B o tn p e r, Margarita liroiiwer. Simon Kind, Neellje Siegh. Cornelis Van den Berg, Elisabeth Pietersse. Abraham Leiiwe, Elisabeth Cregier. O 1 p h e r t Roseveld, Elisabeth Lo n s - berry. Johannes Zuricher, Elisabeth Eensler. Vincent Monlagne, Tryntje Ilartje. Gideon Carsleng, Su- sanna Walgraaf. Abraham Jennet, Maria Gankens.

Antje Rachel. April 2. Robert G. Living- ston, Calharina Mc- Phedris. Henriciis Kip, Hel- ena Low. Laurens Ver Wev, Tryntje de Moon. Jan Am merman, Eva Oiike. Willem Ale. Brandt Schuyler, Margareta Van Wyck. Johannes Hoiiser, Veronika Hoeber.

Michel Smith, Maria Spencer. H a r m e n Coerten, Elsje Sneedeker.

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Sicknick as he lies in honor in the Capitol Rotunda on Feb. Vice President Kamala Harris and the second gentleman Doug Emhoff pay their respects during a ceremony memorializing U. Sicknick, 42, as he lies in honor in the Rotunda of the Capitol on Wednesday, Feb. Capitol Police Officers and other guests are seated during a ceremony memorializing U. A Capitol Police officer pays respects to the late U.

Capitol Police officer Brian Sicknick as an urn with his cremated remains lies in honor on a black-draped table at the center of the Capitol Rotunda, Wednesday, Feb. A member of Congress pays respects to the late U. Capitol Police officer Brian Sicknick as an urn with his cremated remains lies in honor on a black-draped table at the center of the Capitol Rotunda, Feb.

Speaker of the House Nancy Pelosi of Calif. Capitol Police officer Brian Sicknick, as an urn with his cremated remains lies in honor on a black-draped table at the center of the Capitol Rotunda, Wednesday, Feb. Chestnut, who died in the line of duty along with Detective John Gibson on July 24, is comforted after attending the Congressional ceremony for late Capitol Police officer Brian Sicknick as he lies in honor during a ceremony as people pay their respects in the Rotunda of the US Capitol building on Feb.

Trump supporters gather outside the Capitol, Wednesday, Jan. As Congress prepares to affirm President-elect Joe Biden's victory, thousands of people have gathered to show their support for President Donald Trump and his claims of election fraud. A Capitol police officer looks out of a broken window as protesters gather on the U. Protesters attempt to enter the U.

Demonstrators breeched security and entered the Capitol as Congress debated the a presidential election Electoral Vote Certification. Pro-Trump protesters have entered the U. Capitol building after mass demonstrations in the nation's capital. Pro-Trump protesters entered the U. Capitol building during mass demonstrations in the nation's capital.

Capitol police guard a barricaded door as protesters try to break into the House chamber at the U. Capitol on Jan. People shelter in the House gallery as protesters try to break into the House Chamber at the U. Capitol on Wednesday, Jan. Protesters enter the U.

A group of Republican senators said they would reject the Electoral College votes of several states unless Congress appointed a commission to audit the election results. Police keep a watch on demonstrators who tried to break through a police barrier, Wednesday, Jan. Law enforcement officers point their guns at a door that was vandalized in the House Chamber during a joint session of Congress on Jan.

Scott Applewhite, AP. House of Representatives members leave the floor of the House chamber as protesters try to break into the chamber at the U. Jim Jordan, R-Ohio, is at center. Members of congress run for cover as protesters try to enter the House Chamber during a joint session of Congress on January 06, in Washington, DC.

Capitol Police draw their guns as protesters attempt to enter the House Chamber during a joint session of Congress on January 06, in Washington, DC. People wear protective gear as protesters attempt to enter the House Chamber during a joint session of Congress on January 06, in Washington, DC.

People shelter in the House gallery as protesters try to break in at the U. Rioters break through windiws of the U. There are no reports of tear gas being used at the Capitol. Protesters walk as U. House members prepare to leave the floor as protesters try to break into the House Chamber at the U.

Supporters of President Donald Trump breached security and entered the Capitol as Congress tried to confirm the presidential election. Protesters gesture to U. Protesters and police at the Capitol on Jan. Trump supporters try to break through a police barrier, Wednesday, Jan. Trump supporters try to break through a police barrier, Jan. Rioters swarm the U. Supporters of President Donald Trump surround the U. Capitol following a rally on Jan.

Congress meets to formally ratify Joe Biden as the winner of the Presidential election on Jan. We just know it was stolen. We're here to give our voice to the fact that we believe this election was stolen. Thousands of demonstrators gather in Washington, D. Protesters gather in Washington, DC as the U. People listen as Donald Trump Jr. Trump supporters gather on the Washington Monument grounds in advance of a rally Jan.

Trump supporters gather in Washington in advance of a rally Wednesday, Jan. Demonstrators identifying themselves as the "Sons of Liberty, N. Joseph Baker of Cape May, N. Capitol Police officer stands watch on Independence Avenue before dawn as the House and Senate prepare to convene a joint session to count the electoral votes cast in November's election, on Capitol Hill in Washington, Jan. A group with an American flag cross a quiet 12th Street in the direction of pro-Trump rallys on Jan.

Microsoft and partners may be compensated if you purchase something through recommended links in this article. Found the story interesting? Like us on Facebook to see similar stories. These atrocities have gotten to the crescendo before Governor Akeredolu took the right, albeit overdue decision to close the forest reserve of Ondo State to pastoralists activities.

It should be underscored therefore that the inalienable right of Governor Akeredolu to close the forest reserve was very much within the powers conferred on him as the Chief Security Officer of Ondo State. Moreso, the state government had also mandated herders that still wish to continue with their cattle rearing business to register with appropriate authorities within a 7-day window or risk evacuation from the Sunshine State.

The government order had also outlawed night grazing with immediate effect in a bid to stop the herders from continuing with their incessant and unconscionable unleashing of their cattle to feed on hectares of farmlands during the night. After all, an unusual sickness will sure require an unusual cure.

Meanwhile, Governor Akeredolu had unambiguously cited how the activities of armed herders have long been threatening the peace and security architecture of Ondo State. The state government had also done the right thing with the immediate ban of under-aged grazing of cattle as well as the movement of cattle within the cities and highways of Ondo State. These decisions were geared towards checkmating the criminal activities of bad elements amongst the herders domiciled in the Sunshine State.

Some of the disturbing criminal activities of the Fulani herders which necessitated the closure of Ondo forest reserve include the gruesome murder of Mrs Olufunke Olakunrin, the daughter of Afenifere chieftain, Chief Reuben Fasoranti, who was shot and killed by suspected herdsmen along Ore road while going back to Lagos after she had visited had to father in Akure.

Amos Arijesuyo. Also, the former Secretary to the Government of the Federation, Chief Olu Falae had once been kidnapped on his farm in Akure by the same armed herders and made to pay a ransom of N5 million before he could eventually regain his freedom. These high-profile cases, in addition to lots of other under-reported and non-reported ones, were being committed daily by bad elements amongst the Fulani herders in Ondo State as well as other southwestern states.

Disturbingly, instead of empathizing with Governor Akeredolu and his long traumatized people, Garba Shehu had carelessly chosen to hold brief for the Fulani herders. Truly, the challenges plaguing Nigeria may have been fixed even before now, if the presidency had attached much importance to these issues as it would readily give deserved attention to matters that concern Fulani herders.

The presidency never raised any constitutional concern on the matter till day. Garba Shehu may have to tell the nation and indeed the international community if an elected state governor longer has the powers to take crucial and timely decisions that will save his people from further harm and deaths.

The urgency and zealous commitment with which Garba Shehu had replied Governor Akeredolu suggested that the presidency was more interested in the economic interests of Fulani herders rather than that of Ondo State people. These are people that have been getting kidnapped for a huge ransom, brutally killed, and their women raped and infected with different diseases by the same bad elements amongst the Fulani herders daily.

One would have expected the presidency to spare constructive thoughts on why the Fulani herdsmen would turn out the troubler of Ondo State people. Garba Shehu will also need to explain to the nation if the Ondo State Government can no longer make important decisions on her forest reserve asset. He also owes the nation an explanation if the rights of Nigerians to live anywhere is absolute in the face of unmitigated daily threats to the lives and properties of the Ondo State people.

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Capitol Police Officers and other guests are seated during a ceremony memorializing U. A Capitol Police officer pays respects to the late U. Capitol Police officer Brian Sicknick as an urn with his cremated remains lies in honor on a black-draped table at the center of the Capitol Rotunda, Wednesday, Feb.

A member of Congress pays respects to the late U. Capitol Police officer Brian Sicknick as an urn with his cremated remains lies in honor on a black-draped table at the center of the Capitol Rotunda, Feb. Speaker of the House Nancy Pelosi of Calif.

Capitol Police officer Brian Sicknick, as an urn with his cremated remains lies in honor on a black-draped table at the center of the Capitol Rotunda, Wednesday, Feb. Chestnut, who died in the line of duty along with Detective John Gibson on July 24, is comforted after attending the Congressional ceremony for late Capitol Police officer Brian Sicknick as he lies in honor during a ceremony as people pay their respects in the Rotunda of the US Capitol building on Feb.

Trump supporters gather outside the Capitol, Wednesday, Jan. As Congress prepares to affirm President-elect Joe Biden's victory, thousands of people have gathered to show their support for President Donald Trump and his claims of election fraud. A Capitol police officer looks out of a broken window as protesters gather on the U. Protesters attempt to enter the U. Demonstrators breeched security and entered the Capitol as Congress debated the a presidential election Electoral Vote Certification.

Pro-Trump protesters have entered the U. Capitol building after mass demonstrations in the nation's capital. Pro-Trump protesters entered the U. Capitol building during mass demonstrations in the nation's capital. Capitol police guard a barricaded door as protesters try to break into the House chamber at the U.

Capitol on Jan. People shelter in the House gallery as protesters try to break into the House Chamber at the U. Capitol on Wednesday, Jan. Protesters enter the U. A group of Republican senators said they would reject the Electoral College votes of several states unless Congress appointed a commission to audit the election results.

Police keep a watch on demonstrators who tried to break through a police barrier, Wednesday, Jan. Law enforcement officers point their guns at a door that was vandalized in the House Chamber during a joint session of Congress on Jan. Scott Applewhite, AP. House of Representatives members leave the floor of the House chamber as protesters try to break into the chamber at the U.

Jim Jordan, R-Ohio, is at center. Members of congress run for cover as protesters try to enter the House Chamber during a joint session of Congress on January 06, in Washington, DC. Capitol Police draw their guns as protesters attempt to enter the House Chamber during a joint session of Congress on January 06, in Washington, DC. People wear protective gear as protesters attempt to enter the House Chamber during a joint session of Congress on January 06, in Washington, DC.

People shelter in the House gallery as protesters try to break in at the U. Rioters break through windiws of the U. There are no reports of tear gas being used at the Capitol. Protesters walk as U. House members prepare to leave the floor as protesters try to break into the House Chamber at the U. Supporters of President Donald Trump breached security and entered the Capitol as Congress tried to confirm the presidential election. Protesters gesture to U. Protesters and police at the Capitol on Jan.

Trump supporters try to break through a police barrier, Wednesday, Jan. Trump supporters try to break through a police barrier, Jan. Rioters swarm the U. Supporters of President Donald Trump surround the U. Capitol following a rally on Jan. Congress meets to formally ratify Joe Biden as the winner of the Presidential election on Jan. We just know it was stolen.

We're here to give our voice to the fact that we believe this election was stolen. Thousands of demonstrators gather in Washington, D. Protesters gather in Washington, DC as the U. People listen as Donald Trump Jr. Trump supporters gather on the Washington Monument grounds in advance of a rally Jan. Trump supporters gather in Washington in advance of a rally Wednesday, Jan.

Demonstrators identifying themselves as the "Sons of Liberty, N. Joseph Baker of Cape May, N. Capitol Police officer stands watch on Independence Avenue before dawn as the House and Senate prepare to convene a joint session to count the electoral votes cast in November's election, on Capitol Hill in Washington, Jan. A group with an American flag cross a quiet 12th Street in the direction of pro-Trump rallys on Jan.

Microsoft and partners may be compensated if you purchase something through recommended links in this article. Found the story interesting? Like us on Facebook to see similar stories. I'm already a fan, don't show this again. Send MSN Feedback. How can we improve? The resurgent need for an anthrax vaccine s has also refocused concerns regarding the specific efficacy and nonspecific reactogenicity associated with vaccination.

In response to the stated need to develop a well characterized and efficacious vaccine that is also well tolerated [6] , we generated and characterized a novel subunit vaccine that employs C3d as a natural adjuvant and PA as the target antigen for neutralizing antibodies. Production of effector antibodies of all IgG isotypes was sustained, and no evidence of pro-inflammatory cytokines was observed. Second generation anthrax vaccines currently in clinical trials consist of PA adsorbed to aluminum hydroxide.

Protective antigen is an attractive neutralization target since binding, uptake, and cytosolic release of LeTx and EdTx are dependent upon PA. We found that coupling of C3d to PA and emulsion in IFA elicited a rapid PA-specific antibody response in adult and adolescent mice that was superior to the response elicited by equivalent amounts of PA adsorbed to alum or emulsified in IFA. These results could indicate the equivalent generation and survival of PA-responsive B cells from the primary response.

This is an important hallmark, as domains 2 and 4 cooperate to bind the anthrax receptor and thus are indispensable for anthrax toxicity [10]. Despite the fact that aluminum salts have been in use for decades and are the only approved vaccine adjuvants in the U.

Although some proteins adsorb to aluminum hydroxide with high efficiency, several reports have shown rapid desorption in vivo [40]. Such findings argue against a strong depot effect and suggest that adjuvancy may be achieved by the release of concentrated antigen. Microbial pathogens activate the innate immune system by engaging Toll-Like Receptors TLRs , resulting in cytokine release and the upregulation of costimulatory molecules on antigen-presenting cells.

It has been observed that aluminum compounds can promote the maturation or activation of myeloid antigen-presenting cell subsets; however, since TLR signaling does not appear to be involved it is unclear how this occurs [40] , [41] , [42] , [43]. By contrast TLR signaling is thought to be a major contributor to the immunostimulatory activity of complete Freund's adjuvant CFA , consisting of paraffin oil and mycobacterial components. CFA efficiently promotes the maturation and activation of myeloid cells and thus promoting their ability to act as antigen presenting cells for T cells.

By comparison, IFA also promotes the slow release of the immunogen, but nonspecific immunostimulatory activity is minimal or absent in most preparations. We chose IFA emulsion for the work presented here, since we sought to determine the antigen-specific adjuvant activity of C3d in the absence of other costimuli. Nonetheless, parallel studies using PA-Av-C3d 3 in saline would be necessary to formally address the role of IFA in potentiating the effect of C3d conjugation.

In contrast to aluminum hydroxide, the adjuvancy effect of C3d is better understood [35]. Soluble proteins generally do not activate the complement cascade, but need to be opsonized via antibody binding or in the context of local inflammation - as may occur with other adjuvants that activate innate immunity. In addition, C3d fixation facilitates the uptake and presentation of antigen to recruit T cell help [27] , [29].

Therefore, in addition to promoting the recruitment of PA-specific B cells into the primary response, C3d-conjugation promotes the generation of high affinity antibody-secreting cells and the maintenance of the memory B cell compartment. Toxin neutralizing assays revealed similar efficacy with either vaccine and reduced reactogenicity with rPA However, PA-specific antibody titers were significantly reduced in subjects receiving rPA Extrapolating from these findings and our own studies, we conclude that PA-C3d conjugates or fusion proteins hold great promise as a next generation vaccine.

Altogether, our data suggests that PA-linked-C3d would be efficacious as both a preventative vaccine and for the rapid induction of neutralizing antibody upon acute anthrax exposure. Competing Interests: The authors have declared that no competing interests exist. National Center for Biotechnology Information , U. PLoS One. Published online Oct Ravi V. Robert C. Niyaz Ahmed, Academic Editor. Author information Article notes Copyright and License information Disclaimer. E-mail: gro. Received Aug 8; Accepted Sep Copyright Kolla et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. This article has been cited by other articles in PMC.

Abstract B. Introduction Anthrax, an acute infectious disease caused by the spore-forming bacteria B. Purification of C3d 3 -bio and conjugation to PA Cloning of murine C3d and expression of the biotinylated form C3d 3 -bio was described previously [29]. ELISA Peripheral blood was isolated from mice by retro-orbital bleed before immunization and at 7—10 day intervals after immunization. Flow cytometry Single cell spleen cell suspensions were prepared and red blood cells removed by ACK lysis. Histology Spleens were harvested and placed in O.

Mouse challenge with B anthracis Spores were prepared from the Sterne strain of B. Open in a separate window. Figure 1. Figure 2. Figure 3. PA-Av-C3d 3 is an effective immunogen delivered i. PA-Av-C3d elicits strong antibody responses in adolescent mice Another key parameter in vaccine development is safety and efficacy in infants and children. Figure 4. C3d is a functional adjuvant in adolescent mice. C3d 3 augments immune responses by abetting the formation of germinal centers and plasma cell differentiation Germinal centers GCs are specialized structures within follicles wherein B cells undergo somatic hypermutation and class switch recombination of rearranged immunoglobulin genes to generate high-affinity plasma cells or memory B cells [39].

Figure 5. C3d conjugation augments the formation of germinal centers and plasma cells. Figure 6. PA-Av-C3d 3 provides enhanced protection against aerosol anthrax spore challenge. Discussion To avoid a calamitous outcome from B. Footnotes Competing Interests: The authors have declared that no competing interests exist. References 1. Brey RN. Molecular basis for improved anthrax vaccines. Adv Drug Deliv Rev. Surveillance for adverse events associated with anthrax vaccination—U. Department of Defense, — Pharmacoepidemiol Drug Saf.

Development of an improved vaccine for anthrax. J Clin Invest. Turnbull PC. Current status of immunization against anthrax: old vaccines may be here to stay for a while. Curr Opin Infect Dis. Does It Work? Anthrax—an overview. Med Sci Monit. Mock M, Fouet A. Annu Rev Microbiol. Anthrax capsule vaccine protects against experimental infection.

Crystal structure of a complex between anthrax toxin and its host cell receptor. Crystallographic studies of the anthrax lethal toxin. J Appl Microbiol. Crystal structure of the anthrax toxin protective antigen. Immunogenicity in mice of anthrax recombinant protective antigen in the presence of aluminum adjuvants. Evaluation of the compatibility of a second generation recombinant anthrax vaccine with aluminum-containing adjuvants.

Lindblad EB. Aluminium compounds for use in vaccines. Immunol Cell Biol. C3d of complement as a molecular adjuvant: bridging innate and acquired immunity. Immunogenicity and tolerance of ascending doses of a recombinant protective antigen rPA anthrax vaccine: A randomized, double-blinded, controlled, multicenter trial. Vaccine [ PubMed ] [ Google Scholar ]. Characterization of antibody responses to purified HIV-1 gp glycoproteins fused with the molecular adjuvant C3d. Fusion of C3d molecule with bovine rotavirus VP7 or bovine herpesvirus type 1 glycoprotein D inhibits immune responses following DNA immunization.

Vet Immunol Immunopathol. C3d enhancement of neutralizing antibodies to measles hemagglutinin. J Immunol. Local synthesis of C3 within the splenic lymphoid compartment can reconstitute the impaired immune response in C3-deficient mice. Expression of complement receptors 1 and 2 on follicular dendritic cells is necessary for the generation of a strong antigen-specific IgG.

Generation of recombinant human C3dg tetramers for the analysis of CD21 binding and function.

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